An experiment conducted by a researcher at a major university (who wishes to remain anonymous) provides evidence that the amount of the mercury in a vaccine can vary by draw from the same vial. Depending on the location of the draw (top or bottom of vial), as well as whether or not the vial was shaken immediately prior to the draw, the amount of mercury differs.
This had been suspected by consumer-safety-advocates for some time. Concerns were that if correct, and they are, certain vaccine recipients could be receiving more mercury than others. Worse, we wouldn’t know who they were or how much that was.
Consumers of Thimerosal containing multi-dose flu vaccine, for example, are reported to be receiving 25-micrograms of mercury per injection. This is based on the assumption that each draw from the vial contains a consistent amount of 25-micrograms, provided that all vaccine vials are shaken immediately prior to being drawn, thereby evenly distributing the 250 mcg in every 5-mL vial that holds ten 0.5-mL draws.
But, the researcher wondered, what if that’s not the case? What if someone forgets to shake it? What if a vial sits overnight or for say, twenty minutes? And does it matter from where the draw is then taken (top or bottom of the vial), shaken or not?
As this experiment shows, it does. Here’s how it was done.
Laboratory Method for Mercury Analysis of Vaccines
A 5-mL sample of multi-dose Flulaval flu vaccine by GlaxoSmithKline was obtained.
To test for mercury content, all sample manipulation was conducted under particle-free conditions in exhausting laminar flow hoods equipped with HEPA filters.
Three subsamples were obtained and analyzed from the 5-mL multi-dose vaccine. Subsamples were drawn from the top, bottom, and bottom-after-settling for 20 minutes, respectively. The entire content of each sample was drawn into a 1 mL rubber-free plastic syringe with a 23-gauge stainless steel hypodermic needle.
Sample volumes of 0.5 mL were estimated using the graduation on the 1-mL syringes. The content of the syringe was dispensed into a trace metal free glass bottle prefilled with 450 mL mercury-free water and 2.25 mL BrCl solution (preparation according to EPA Method 1631).
The samples in glass bottles were periodically shaken and stored overnight to allow the oxidation of mercury. Bottle contents were further diluted as necessary then analyzed using a Tekran 2600 automatic mercury analyzer via Cold Vapor Atomic Fluorescence Spectrometry (CVAFS) with dual preconcentration.
After allowing a 5-mL vial of flu vaccine (from which two draws had already been taken) to settle for only 20 minutes after being shaken and then pulling a third 0.5-mL draw from the bottom of the vial, the content of the mercury in the draw had increased. Instead of 25 mcg, it had 27.
The finding is significant.
One, it provides evidence that mercury does in fact settle at the bottom of the vial. And although a 2-microgram increase may not seem like a big deal, consider this. In the first draw, 25 mcg represents 10% of the 250-micrograms of total mercury in the vial. But, in the third draw, 27 mcg represents 13.5% of the remaining 200-micrograms of mercury in the vial. That’s over a 25% increase.
Two, it weakens the epidemiology done on the relationship between autism and Thimerosal even further. Those studies, which are the only kind of studies being used to debunk “the mercury myth”, as well as upon which the Institute of Medicine relied exclusively to dismiss the hypothesis, are based on research that assumed precise, consistent amounts of Thimerosal per draw and per child in the studies they used.
That assumption was wrong. Because…
Three, as this experiment shows, we will never know exactly how much mercury a child actually received. Parents who have taken comfort in believing their child received “only” 25-micrograms, per se, actually have no idea, and never will, what their child was truly injected with. Did they get a draw from the bottom? Was the jar shaken? How much prior to the draw was it shaken?
No one knows.
Four, it provides yet one more reason Thimerosal belongs absolutely nowhere near a human being. Besides being a neurotoxin that has never been tested for safety, the argument the dose makes the poison simply doesn’t fly when you consider…
They don’t actually know the dose they are giving.
Julie Obradovic is a Contributing Editor to Age of Autism.