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A Proposed Laboratory Neuron Toxicity Test Model for Thimerosal

NumbersBy Jim Thompson

The following is a mathematical model based on curve fitting of data from Baskin et al (2003) from cultured human brain cells exposed to Thimerosal in a laboratory environment.  It does not represent data observed for brain cells within a human body.  The purpose of this toxicity model is to illustrate how the Baskin study might be used to provide guidance for further study and offer insights into the potential for Thimerosal preserved vaccines toxicity to human brain cells when administered to children. 

The mathematical model is based on the following:

The Thimerosal preserved flu shot recommended by the FDA and CDC for 6 month old children and pregnant women has approximately 25 micrograms (ug) of mercury.  Currently there are an estimated 83 million flu vaccine doses preserved with Thimerosal. 

The human brain has an estimated 100 billion brain cells.
Ball et al estimated a median weight for 6 month old females to be 7.21 kilograms.  See page 1150. 

The whole body weight-weight ratio of mercury after a single flu shot is calculated at 25 ug / 7.21 kg or 3.47 parts per billion.  This is a 17.3 mercury nanomolar concentration.

Baskin et al measured a failure rate of laboratory cultured human neurons, after 6 hours, as follows: "Assessment of 200 cells per well randomly, using the fluorescent microscope, revealed that active caspase-3 was expressed in 20% of the cells at 2 uM [micro molar concentration] thimerosal, 26% at 10 uM thimerosal, 83% at 50 uM thimerosal, and 97% of the neurons at 250 uM thimerosal concentration. In the controls, less than 1% of the cells was caspase-3–positive, due to cell death naturally occurring in the cell cultures." See Baskin et al, 2003, p. 365,.

Using Mathcad 2001 Professional a non-linear, mono-exponential regression model (see Equation 1) was made using the Mathcad least squares solution method called Minerr.  Data was taken from the Baskin reported laboratory cultured brain cell failure rate at a 6 hour exposure time referenced above.  While the overall Baskin results were limited to a minimum level of toxicity of one micromolar (uM) of mercury concentration for human neuron damage in a cultured laboratory experiment, the authors stated that “These results indicate that additional research is needed to more fully delineate the dose- and time-dependent toxicity of thimerosal in sub-micromolar concentrations…” 

Consequently the following equation is proposed as a model of the failure rate, at a 6 hour exposure time, for cultured neurons in a laboratory environment after extrapolation from a micromolar concentration range of mercury exposure down to a nanomolar (nM) concentration range of mercury exposure and for the number of neurons approaching that estimated in the human brain:

Click to enlarge.

Eq. 1 Cultured Neuron % failure rate for nanomolar Thimerosal mercury exposure at 6 hours
The failure rate at the whole body burden value of 3.47 parts per billion Thimerosal based mercury (17.3 nM), equivalent to the whole body exposure for a 6 month old girl receiving a Thimerosal preserved flu vaccine, is 0.06 percent.  Multiplying the number of brain cells in a human (100 billion brain cells) times the model percent failure rate for 3.47 parts per billion Thimerosal mercury (17.3 nM) yields approximately 60 million brain cell failures for a 6 hour exposure time.

It is important to note that this is a proposed model for a laboratory study of cultured neurons.  The mercury levels and the neuron failure rates in the brain of a child or a pregnant woman’s fetus 6 hours after receiving a flu shot with Thimerosal preservative are unknown. 

None-the-less without information on mercury exposure levels to brain cells, the time of exposure, and estimates of the neuron failure rate in the human body-- the principle of “first do no harm” seems logical and a ban of all mercury from vaccines seems necessary for the health of our children.

Jim Thompson is a registered professional engineer.  He and his wife Susan live and work in rural South Dakota.  Their first granddaughter Taylor Haug was diagnosed with autism spectrum disorder, epilepsy, verbal apraxia, motor disorder, and sensory integration disorder. Her loving memory has influenced his family’s decision to help protect children from vaccine injuries. 


Taylot Haug




Barbara G. Dan

My grandson (born 11/23/1993) was given MMR at 15 months. Up till then he was outgoing, highly verbal, physically and mentally alert. Within 2-3 weeks after the MMR shot, he was in another world, not reacting, not hearing when called, etc. Diagnosed shortly thereafter by a pediatrician as having severe autism. In his 20s now, he is severely impaired.

I hear there have been hearings about the CDC cover-up in Autism Omnibus Proceedings.
My grandson's father (my son) is currently in law school and hopes to find the court cases related to the CDC. Since predictions are that by 2025 all American children will have autism, we are hoping to know the actual Court Cases and Nos. so more research into this can be done. Any help you can provide will be deeply appreciated so CDC can be held accountable.. Somehow this tragic loss of children to autism MUST be reversed!

Jim Thompson


Scientists have known for over 70 years that mercury nanomolar concentrations are toxic. A study from 1939 reported mercury chloride in water concentrations of 10 parts per billion (50 nano-molars of mercury) to be lethal for Stickleback fish at an exposure time of 8 ½ days. See Jones (1939), figure 1 at http://jeb.biologists.org/content/16/4/425.full.pdf .

Researchers at the National Institute for Minamata Disease have recently shown in laboratory animal brain cell cultures, using 100 nanomolar concentrations of methyl mercury, that methylmercury is 100 times more neurotoxic than lead. This was based on a “concentration responsible for 30% loss of viability (EC30) values 3 days after exposure.” See Fujimura and Usuki (2012) http://www.ncbi.nlm.nih.gov/pubmed/22223485

And animals with intramuscular injections of Thimerosal at 12 parts per billion mercury weight-weight ratio (60 nanomolars ethylmercury concentration) are reported to have significant neuron failure. See Olczak (2010), “Lasting Neuropathological Changes in Rat Brain After Intermittent Neonatal Administration of Thimerosal.” at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2957583/pdf/11064_2010_Article_250.pdf


Thank you for your efforts Mr. Thompson, I miss a few math lectures but looks good to me...

Is there a formula to calculate the "fraud and stupidity" in using something banned in vaccines in other countries for over 20 years ???

Jim Thompson

Kristina, that is correct. So the proposed laboratory model, if based on two doses (0.25 milliliters with 12.5 micrograms of mercury each at four weeks apart at six months and seven months old, instead of 0.50 milliliters with 25 micrograms of mercury given at six months old) gives a slightly smaller cumulative result in the proposed model—that is approximately 59 million laboratory neuron failures rather 60 laboratory million neuron failures.
See the growth chart at http://www.cdc.gov/growthcharts/data/who/grchrt_girls_24lw_9210.pdf

Mary Tocco

If we don't recommend eating thimerosal...why do we recommend injecting it? Seems pretty simple to me. Thimerosal on the skin is deadly.

I believe we all know that any form of thimerosal or mercury does not belong in the human body and yet scientists are on the defensive to "prove" it is harmful instead of the vaccine manufactures "proving" to us it is safe!

Einstein said "No amount of experimentation can ever prove me right; a single experiment can prove me wrong." Doing the 1 study: Comparing the Un-vaccinated to the vaccinated would put this whole issue to rest!


Children 6 months to 35 months are given half the adult dose (0.25 mL instead of 0.5 mL). However, the first year they get the flu vaccine, they are given 2 of those half doses, at least 4 weeks apart. After that, it is just one 0.25 mL dose, until age 36 months, when they get the full adult dose.

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