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Vaccines and Autism – Answering the Wrong Questions

Autism Linked to Increased Genetic Change in Regions of Genome Instability

Science post imageRead the full report at Science Codex.

Children with autism have increased levels of genetic change in regions of the genome prone to DNA rearrangements, so called "hotspots," according to a research discovery to be published in the print edition of the journal Human Molecular Genetics. The research indicates that these genetic changes come in the form of an excess of duplicated DNA segments in hotspot regions and may affect the chances that a child will develop autism -- a behavioral disorder that affects about 1 of every 88 children in the United States, according to the Centers for Disease Control.

Earlier work had identified, in children with autism, a greater frequency of rare DNA deletions or duplications, known as DNA copy number changes. These rare and harmful events are found in approximately 5 to 10 percent of cases, raising the question as to what other genetic changes might contribute to the disorders known as autism spectrum disorders.

The new research shows that children with autism have -- in addition to these rare events -- an excess of duplicated DNA including more common variants not exclusively found in children with autism, but are found at elevated levels compared to typically developing children. The research collaboration includes groups led at Penn State by Scott Selleck; at the University of California Davis/MIND Institute by Isaac Pessah, Irva Hertz-Picciotto, Flora Tassone, and Robin Hansen; and at the University of Washington by Evan Eichler.

The investigators also found that the balance of DNA duplications and deletions in children with autism was different from that found in more severe developmental disorders, such as intellectual disability or multiple congenital anomalies, where the levels of both deletions and duplications are increased compared to controls, and are even higher than in children with autism.

They also found that children who had more difficulty with daily living skills also had the greatest level of copy number change throughout their genome. "These measures of adaptive behavior provide an indication of the severity of the impairment in the children with autism. These behaviors were significantly correlated with the amount of DNA copy number change," Selleck said, emphasizing that the research revealed "clear and graded effects of the genetic change."

These microscopic images were taken as part of research to explore rearrangements of DNA in one of the "hotspots" of the human genome, where deletions and duplications occur at higher rates. More information is online at science.psu.edu/news-and-events/2013-news/Selleck4-2013.

 

"These results beg the question as to the origin of this genetic change," Selleck said. "The increased levels of both rare and common variants suggests the possibility that these individuals are predisposed to genetic alteration."

A vigorous debate is ongoing in the research community about the degree of genetic versus environmental contributions to autism. Selleck said the finding of an overall increase in genetic change in children with autism heightens the need to search for the basis of this variation. "We know that environmental factors can affect the stability of the genome, but we don't know if the DNA copy number change we detect in these children is a result of environmental exposures, nutrition, medical factors, lifestyle, genetic susceptibility, or combinations of many elements together," Selleck said. "The elevated levels of common variants is telling us something. It suggests that pure selection of randomly generated variants may not be the whole story."   Read the full story at Science Codex,

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Lowell

Spontaneous Integration of Human DNA Fragments Into Host Genomes

Results:

Spontaneous cellular and nuclear DNA uptake was evident in HFF1 and U937. Spontaneous cellular uptake was seen in NCCIT. DNA uptake in BE (2)-C, M059J, and M059K was not measurable because of high auto fluorescence of the cells. No Cy3 signal was observed in HL-60. The amount of labeled Cy3 human Cot1 DNA incorporation in U937 genomic DNA was 0.0111 +/- 0.0034pg (n=12) per cell in 24 hours, which was approximately 0.167% of total U937 genomic DNA.

Conclusions:

This study demonstrates that primitive short DNA fragments (50-300 bp) are spontaneously taken up by HFF-1, U937 and NCCIT cells and inserted into the genome of the monocytic leukemia cell line U937. Hence, vaccines containing residual HERVK and human fetal DNA fragments may contribute to the genomic instability observed in ASD.

http://soundchoice.org/wp-content/uploads/2012/08/DNA_Contaminants_in_Vaccines_Can_Integrate_Into_Childrens_Genes.pdf

Computational Detection of Homologous Recombination Hotspots in X
http://download.pdf-world.net/Computational-Detection-of-Homologous-Recombination-Hotspots-in-X-download-w4554.html

For Scientific Data: Homologous Recombination Study
http://www.cogforlife.org/SCPIIMFARHR.pdf

Autism Research

The scientific community now knows that children with regressive autism have hundreds of de novo and diverse gene mutations. That means that regressive autism is not genetic. It must be triggered by an external event that can create
hundreds of different DNA breaks and mutations. That has been obvious to us at SCPI since we were founded.

Since 2008, SCPI has been ahead of the field, doing the cutting edge biology, molecular modeling, computational informatics, and ecology to figure out what is causing so many DNA breaks and mutations in our children, long before the 2011 and 2012 publications put the hundreds of de novo mutations together. That’s because we knew that a condition associated with hundreds of different genes could not possibly be genetic. We knew that DNA and retroviral contaminants are present in some childhood vaccines and that these types of contaminants are known to cause DNA breaks and mutations.

Have we created the perfect storm for DNA breaks, mutations, and regressive autism in our children? In 1979 we started injecting our children with vaccines that are contaminated with aborted fetal DNA fragments and a retrovirus, and autism began to rise. Then we added more jabs with aborted fetal vaccines and thimerosal, which can also cause DNA breaks, to vaccines in 1988, and autism rose more. Then in 1995, we added much more aborted fetal DNA contaminants to the chickenpox vaccine, and autism really rose. And now we have children born to older dads who have sperm with very breakable DNA. Aborted fetal contaminated vaccines plus thimerosal plus older dads result in more DNA breaks, thus more de novo mutations, in our children.

Read more:
http://soundchoice.org/research/

Vaccine Production With - Human Diploid Cells (aborted fetal cell tissue)
http://www.vacfacts.info/vaccine-production-with---human-diploid-cells-aborted-fetal-cell---tissue.html

Lou

"There is no brain disease, which is caused purely by genetic factors."

I am reminded of the idiocy of many PhDs by the SERIOUS debate that seemed, to us laymen, to rage in in the genetic community over the "genetic" cause of homosexuality.

If we humans chose our leaders with the same precision as our genome constructs our bodies there would be no war, no crooked politicians, little disease and NO AUTISM. When I was a kid only US Senators were millionaires. Today MANY US Congressmen are millionaires. MOST politicians are honest.

Autism is a 100% political disease. It is bought by those pushing the UN Agenda 21 Depopulation Mandate and other crap. It is paid for by we the parents of our kids, our society, and our kids, who are being killed, grievously harmed, harmed and in other ways molested by our political process.

Lou

"A vigorous debate is ongoing in the research community about the degree of genetic versus environmental contributions to autism."

Yes I am sure HHS can fund this "debate" for a few more decades. Meanwhile the rate of autism is doubling every 3.5 years.

How about a simple study or two of "vaccinated" vs unvaccinated children?

Oh wait such a study done on millions of children has been completed for us.

"In New Delhi, India, prior to 2000, ASD/PDD (autism spectrum disorder/pervasive developmental disorder) symptoms were rare – typically only occurring in children who were vaccinated abroad. However, after the Indian pediatricians began recommending, in 2000, the addition of triple-dose Thimerosal-preserved Hib (Haemophilis influenza B) and Hep B (hepatitis B) vaccination programs to the existing Thimerosal-preserved triple dose DTP (diphtheria toxin, tetanus toxin and pertussis toxins) vaccination program recommended by the Government of India, the incidence of a childhood ASD/PDD diagnosis increased to 2 % to 4 % of vaccinated New Delhi children.” Doctor Paul King PhD

"To all but those who worship the god “vaccine” and nowingly sacrifice others children on this god’s altar, these 9 Thimerosal-preserved vaccine doses are the cause of the chronic disease epidemic. After reading this declaration, this reporter challenges any one to say the cause is not the Thimerosal-preserved vaccines and/or that the epidemic of chronically ill children that has been engulfing us since the late 1980s and India since the early 2000s has no cause." Doctor Paul King PhD

http://dr-king.com/docs/100711_ParallelsinNewDelhiIndia_AnEpidemic_b.pdf

Serena

Spontaneous Integration of Human DNA Fragments into Host Genome:

http://soundchoice.org/wpcontent/uploads/2012/08/DNA_Contaminants_in_Vaccines_Can_Integrate_Into_Childrens_Genes.pdf

nd

@Jane Green

Thanks for the link to the UC Davis MIND Institute epigenetics symposium--but aren't the effects that were discussed there essentially PREnatal?

I mean, doesn't it suggest that to have an effect throughout the body (or the brain) the epigenetic changes must affect more than a minority of cells? The site discusses "ancestral germ cells" and "prenatal exposures" and suggests that "we now know that non-genetic heritability can result from environmentally-induced epigenetic changes in the germ cells (sperm or egg)" and "germ cells [are susceptible] to environmentally-induced epigenetic perturbations, particularly at certain windows" and "prenatal EDCs [endocrine-disrupting compounds] interfere with natural hormone activity and may interfere with the hormonal role in sculpting the fetal nervous system and
brain"

Isn't this just more of the same? Where do vaccines come in?

no vac

There is no brain disease, which is caused purely by genetic factors. All are induced by some environmental insults. In case of autism, it is the toxic, excessive and too early vaccines.

Jeannette Bishop

"'These results beg the question as to the origin of this genetic change,' Selleck said."--no kidding, but then...

"The increased levels of both rare and common variants suggests the possibility that these individuals are predisposed to genetic alteration."--or their environment predisposes them to genetic alteration.

nhokkanen

Of course the DNA damage has nothing to do with government-mandated injections of mutagens....

Garbo

I can't tell if these researchers are content to keep beating around the bush and collecting their grants, or just moving at a snail's pace to gingerly lay the foundations for the eventual "ta da!" moment when they are finally ready to use the V word in the same sentence as the A word. Do you suppose they are EVER going to get around to investigating whether these complex and "inexplicable" mutations are in any way related to toxic metals, disrupted methylation and immune systems, and recombination with HERV/human DNA contamination found in the live virus vaccines? Seriously, it's starting to feel like we're sitting in a crowded movie theater where the whole audience knows what's going to happen next, but the handsome yet apparently rather dim hero hasn't quite figured it out yet. Boring.

Jane Green

See www.autismepigenetics.org (project of Autism Speaks and UC Davis MIND Institute) for more on this theme of environmentally induced changes of gene function in autism.

Benedetta

Cutting through the deceit of fancy talk; all they needed to say is: These kids have been poisoned.

But that is not their game.

If they can hold out long enough, if the numbers of kids that show symptoms of the poison can be held at -- well I am not sure what percentage they have decided on - since it has gone from 1% to 2% -- how high do you think they are willing to go?

But if they can hold out -- they can rid the world of a lot of diseases just like they rid it of small pox.

Notice I did not include polio-- because I ran into a little paralyzed girl whose grandparents and her are raising show chickens. She suddenly came down with something that looks like polio to me but the docs called it Transverse myelitis

nd

Greg, the DeStefano article is available here:

http://jpeds.com/webfiles/images/journals/ympd/JPEDSDeStefano.pdf

It looks as if your questions about vaccine dosing might be addressed at the upcoming International Meeting for Autism Research:

https://imfar.confex.com/imfar/2013/webprogram/Paper12796.html

Linda

Part 2 of my post above, a quote from The BioInitiative Report, p. 22 of Section 20 by Martha Herbert, PhD, MD and Cindy Sage MA:

"Genotoxicity

One route through which environmental impacts may influence an organism’s status is by changing genes through mutation – that is, by genotoxicity. This has been proposed as a
mechanism for the generation of ‘de novo’ mutations (found in children but not their parents) being found in ASDs (Kinney et al. 2010) and increasingly in other settings as
well, making mutations something that needs to be accounted for rather than simply assuming tey are associated with normal, stable variation. Reviews and published scientific papers on genotoxicity and EMF report that both ELF-EMF and RFR exposures can be considered genotoxic – i.e., damaging to DNA – under certain conditions of exposure, including under conditions of intermittent and/or chronic ELF and RFR
exposure that are of low-intensity and below current world safety standards (Ruediger 2009; Ivancsits et al. 2005; Diem et al. 2005; Blank and Goodman 2011; Phillips, Singh,
and Lai 2009; REFLEX 31 May 2004; Sage and Carpenter 2009; Lai and Singh 2004). Types of genetic damage reported have included DNA fragmentation and single- and double-strand DNA breaks, micronucleation and chromosome aberrations, all of which indicate genetic instability. Genotoxic impacts of EMF/RFR are further reviewed in the BioInitiative Working Group 2007 contribution by Lai as well as in Section 6 of the present Bioinitiative Report {Lai, 2007; Lai, 2012). The European research program REFLEX (Risk Evaluation of Potential Environmental Hazards From Low-Energy Electromagnetic Field Exposure Using Sensitive in vitro Methods – a 5FP EU project) documented many changes in normal biological functioning in tests on DNA at exposure levels below existing public safety standards(REFLEX 31 May 2004). Some of the key findings included:

• Gene mutations, cell proliferation and apoptosis which are caused by or result in altered gene and protein expression profiles. The convergence of these events is required for the development of all chronic diseases.

• Genotoxic effects and a modified expression of numerous genes and proteins after EMF exposure could be demonstrated with great certainty.

• Genotoxic effects produced by RF-EMF in fibroblasts, HL- 60 cells, granulosa cells of rats and neural progenitor cells derived from mouse embryonic stem cells.

• Response of cells to RF exposure between SAR levels of 0.3 and 2 W/Kg with a significant increase in single- and double-strand DNA breaks and in micronuclei frequency.

• A clear demonstration of increase in intracellular generation of free radicals in
HL-60 cells accompanying RF-EMF exposure.

• The observation that the induced DNA damage was not based on thermal effects,
which raises concerns about the thermal-based environmental safety limits for
ELF-EMF exposure.

These impacts could be contributors to a role for genetics in ASDs that does not derive from only inheritance but also from environmental and epigenetic influences. Moreover,
in the light of the great heterogeneity of genetic findings in ASD alongside the documented impacts of EMF/RFR upon many other levels of pathophysiology than simply genetics, it becomes worth reflecting whether genetics might not be the primary problem but instead, in many cases at least, just one of many levels of collateral damage from environmental impacts. Whatever genetic variants a person carries may bias their system toward specific vulnerability, or may contribute more generically by increasing entropy and molecular disorder; in either capacity they may aggravate the situation but may not be part of the main cause."

There are recommendations in the Report for steps to take to minimize exposures.

Linda

Genetic changes have been found to be caused by electromagnetic fields (EMF) and radio frequency radiation (RFR). Please see "The BioInitiative Report", originally published in 2007 and updated December 2012 http://www.bioinitiative.org/. Section 20 of the report, "Findings in Autism (ASD) Consistent with Electromagnetic Fields (EMF) and Radiofrequency Radiation (RFR)" is by Martha Herbert, PhD, MD and Cindy Sage, MA. I'm quoting 2 sections here (in 2 separate posts - caps, my emphasis) but please see the report for further information and context:

p.9-10 of section 20:

"3. Pathophysiology and allostatic load

Based on these considerations, the strategy to be pursued in this examination of a potential EMF/RFR - ASD link is to review the many parallels between underlying biology, or pathophysiology, in ASDs and the impacts of EMF/RFR on living organisms. EMF/RFR EXPOSURES HAVE DEMONSTRATED IMPACTS AT JUST ABOUT EVERY LEVEL AT WHICH BIOLOGY AND PHYSIOLOGY HAVE BEEN SHOWN TO BE DISRUPTED IN ASDs. EMF/RFR has been shown to potentiate the impact of various toxicants when both exposures occur together (Juutilainen, Kumlin, and Naarala 2006); this may be additive or more than additive. This suggests that EMF/RFR may synergize with other contributors and make things worse. With many different environmental factors piling on to a much smaller number of environmentally vulnerable physiological mechanisms (Herbert 2010), one must consider that the model of ‘allostatic load’ – the sum total of stressors and burdens – may be central to understanding how the many risk factors interact to create autism – and to create a spectrum of levels of severity across so many of ASD’s associated features. A cascade of exposures interacting with vulnerabilities can potentially lead to a tipping
point for an individual, such as the phenomenon of autistic regression experienced by a substantial subset of people with ASDs. When exposures increase at the population level, we are likely to see trends of increase in the number of people passing that tipping point and getting diagnosed. EMF/RFR EXPOSURES HAVE INCREASED SEVERAL THOUSAND-FOLD OR MORE IN THE PAST TWO DECADES FROM WIRELESS TECHNOLOGY INNOVATIONS THAT HAVE UNPLANNED SIDE EFFECTS FROM PULSED RFR, A NEWLY CLASSIFIED HUMAN CARCINOGEN (Baan et al, 2011). Nearly six billion people globally own wireless phones, for example. Many hundreds of thousands more are exposed to wireless whole-body transmissions from wireless antenna facilities (Sage and Carpenter, BioInitiative 2012 Report, Section 24). For this as well as for physiological reasons allostatic loading as a viable concept for the study of ASDs should reasonably address EMF/RFR as one of the collection of exposures of relevance to the overall stress load, since it is now a chronic and unremitting exposure in daily life at environmentally relevant levels shown to cause bioeffects from preconception and pregnancy through infancy, childhood and the whole lifecourse."

cmo

They will do ANY STUDY EXCEPT

the simple vaxed/ unvaxed study requested by Congress in 1999 to see if the Autism rates for the two groups are the SAME.

Greg

Thanks Katie,

A wonderful summary of the latest news on the autism front. Regarding the vaccines study, I am particularly interested in why they did not report the results of how the control and autism groups compare on number of vaccines received or doses. I have been asking this question numerous times without getting an answer. On another site, I did come across a pro-vax blogger that had lots of info about the study, but when I asked him the same question he quickly ran away.

In the method section of the study they provided charts making it clear that along with counting antigen amounts they also counted doses. There have been a lot of assumptions that the two groups received the same amount of vaccines, but if this were the case why would they have needed to count doses. Second, nothing in the study makes it clear that all the subjects received the same amount of vaccines or doses; in fact, quite the opposite and I provide these two quotes from the study:

"We obtained the children’s vaccination histories from computerized immunization tracking systems and abstracted medical charts."

"Some of the case children, however, might have exhibited indications of neurodevelopmental problems well before receiving an ASD diagnosis. How evidence of early neurodevelopmental delays would have affected our results is not clear; it might have resulted in lower vaccination levels if parents were concerned about vaccinating their children, or possibly higher
vaccination levels through more frequent contact with the healthcare system."

In the first quote, they mentioned that the subject’s vaccination history was obtained from a immunization tracking system, which does not indicate conformity. In the second quote, it explicitly discussed the possibility that some of the children may have had less or more vaccines. My point is it seems they had the dose comparison figure but they did not provide it. This study has the feel of after detecting the 'horrific' result that the control group had fewer vaccines -- hence proving our point -- they quickly settled on reporting on only antigen amounts.

Greg

PS: Anyone has any ideas on how to go about getting more info on the study?

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